Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm

Toll‑like receptor (TLR) 2/4 serves an important regulatory role in nerve tissue injury. However, the downstream and potential mechanisms remain to be elucidated. The present study was designed investigate roles of TLR2/4‑major myeloid differentiation response gene 88 (MyD88)‑NF‑κB signaling pathway development intracranial aneurysm. expression TLR2, TLR4 MyD88 blood normal controls patients with aneurysm were detected by quantitative PCR ELISA. Human brain vascular smooth muscle cells treated Angiotensin II (Ang II) evaluate involvement TLR2/4‑MyD88‑NF‑κB process. in vitro experiment divided into four groups: control group, Ang Ⅱ + small interfering (si)RNA group TLR2‑group. Cell viability, migration, apoptosis TLR4, MyD88, NF‑κB phosphorylated (p‑)p65 detected. results demonstrated that at mRNA protein levels significantly higher compared corresponding (P<0.05). In experiments treatment increased cell proliferation migration rate but reduced apoptotic p‑p65 (vs. control; P<0.05). By contrast, TLR2‑short RNA rate, short In conclusion, data indicated is involved pathogenesis